Requires the blackworms to incubate 2 min in the test solution. If the worms were accidentally incubated for 4 min, how would the results be affected?

a. The results would not be affected since the test chemicals are known to not affect the pulse rate of blackworms.

b. The results would not be affected since an incubation time of 2 min is simply a time-saving device.

c. The pulse rate would be markedly increased due to excessive absorption of the test solution.

d. The pulse rate would be severely depressed or absent due to death of the worms.

Blackworms are organisms most effeciently used by scientists to study the effect of drugs on the circulatory system. They have a transparent skin which make it easier to observe pulse rate. Drugs easily diffused through the skin of blackworms which cause immediate effect and they are easy to maintain in the laboratory. When the blackworms are incubated with extra 2 minutes, their pulse rate will increased due to excessive absorption of the test solution.